Biography & Net Worth: Gallic acid and stretch reduce chondrocyte osteoarthritis markers

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A healthy diet and a little exercise seem to be good for arthritis on a cellular level as well.

A team led by Washington State University researchers applied a stretching mechanism to humans using gallic acid, an antioxidant found in green tea and other plants. Cartilage cells taken from the knee with arthritis that mimic the strain that occurs when walking. This combination not only reduced the inflammatory markers of intracellular arthritis, but also improved the production of desired proteins normally found in healthy cartilage.

Although still in its infancy, the findings suggest that new procedures may be developed to increase the supply of cells or tissues for re-implantation of chondrocytes extracted from patients.

“We found that stretch and gallic acid, which act like the movement of cells themselves, reduced inflammatory markers, meaning they were able to reverse osteoarthritis,” the study said. said Hanin Abushark, lead author and most recently lead author of WSUph.D. .. Graduation. “It’s basically like getting good exercise and a good diet on a micro level.”

for research experimental cell researchResearchers harvested osteoarthritis chondrocytes from donated knees inside a joint replacement at Pullman Regional Hospital. They cultured cells in the lab and tested the first six antioxidants, “dietary supplements,” namely nutritional products such as vitamin C, vitamin E and curcumin. Antioxidants can neutralize free radicals, which are unstable atoms that result from oxidative stress that can damage cells and tissues.

Laboratory Testing We have proposed gallic acid as the most effective antioxidant for neutralizing free radicals of chondrocytes in osteoarthritis. Subsequently, a site stretcher developed by Curie Bio Inc. was used to apply gallic acid and add stretch. The stretch was set at 5%, a level consistent with human knee strain during walking.

This combination reduced an inflammatory marker known as matrix metalloproteinase. Increased deposition of collagen and glycosaminoglycans. These are compounds that give connective tissue its integrity, tensile strength and resistance to compressive forces of joint loads. Stretching and gallic acid also increased the expression of two other cartilage-specific proteins.

Osteoarthritis, the most common musculoskeletal disorder in the world, destroys the cartilage of the joints, causing pain and limiting movement. Currently, there is no complete cure, from prescribing painkillers to surgically replacing the joints with synthetic joints, but even surgery does not allow patients to return to their full range of motion. .

Another procedure, called autologous chondrocyte transplantation, or ACI, involves removing chondrocytes from joints, growing them in large numbers, and then transplanting them again. Currently, cells are not treated before re-transplantation, and the lack of treatment results in cell growth. Weak fibrocartilage. They can also be affected by osteoarthritis, and these procedures do not return full function to the joint. This study shows possible ways to develop similar processes by treating them first. Chondrocytes can be grown in tissue and then reimplanted.

“We are advancing technology to create regenerated cartilage that can be implanted into cartilage lesions in the laboratory, reducing the number of joint replacements,” said Voile and the School of Chemical Engineering and Bioengineering. WSU professor Bernard Van Wee said. Principal investigator and corresponding author. “Our goal is to develop natural cartilage that works properly from the beginning, rather than replace the joints.”

This study adds to evidence that eating foods high in antioxidants and exercising may be better, but researchers shouldn’t consider gallic acid to be a miracle cure and a treat at all. Also warns that this should be done only in consultation with an individual.

“This provides some evidence that good diet and exercise really do work,” Abshark said. “Even in people with mild osteoarthritis, exercising is really good. Lying or sitting all day is very bad for the cartilage tissue. It requires little activity.”

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for more information:
Hanin A. Abushark et al, a combination of stretching and gallic acid, lowers the inflammation index and promotes extracellular matrix production in human articular chondrocytes with osteoarthritis. experimental cell research (2021). DOI: 10.1016/j.yxcr.2021.112841

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Washington State University

CitationGallic acid and extension reduce chondrocyte osteoarthritis markers (December 15, 2021).

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